Each student will be allocated patient case notes and a sputum sample on day 1.The task is to perform an identification and sensitivity testing within 72 hours (practical
days 1-3) using a workflow similar to a standard diagnostic lab.
This will require you to:
1. Identify the organism involved using appropriate media, workflow, biochemical tests,
and anti- microbial sensitivity testing.
2. Write a brief clinical report to the physician on your findings. A reporting template
will be provided.
3. Write a report on your case study and identification. You are required to describe
the method, workflow and results of your identifications, and to discuss your
findings, and the anti microbial sensitivity findings. In addition, you will research the
symptoms, pathogenesis and treatment of the pathogen you identify.
The following headings should be used for your Case Study report:
• Cover sheet
• Materials and Methods
• Laboratory Report
• References (Vancouver format).
• Laboratory Notes- attach a scanned copy of the notes from your laboratory
Further details on what to include under these headings are given below.
Please note correct formatting and referencing are part of the assessment criteria
and marks will be deducted if format is not correct.
Please include a cover sheet with your name, student number, practical group and
demonstrator, the name of your practical partner, and the identification code of your
Note: The methods section of your report will probably be very similar so other
students- this is not a problem.
The introduction should include general background information including: the
significance infections of the particular body system being studied, reasons for
performing microbiological investigations on these patients, and expected outcome
or benefit from such investigations.
The introduction should end with a brief statement that summarises the aim of the
exercise and nature of the case that was investigated.
Materials and Methods
The protocol used in your own investigations should be explained and justified, this
should be written in the past tense. e.g. “To identify Salmonella spp. to the level of
serotype, the following tests were performed. . .”.
Methods section should be written so that the work could be repeated by another
For this type of report, you do not need to list materials used as these are standard
and may be found in any microbiology laboratory. Any test procedures described in
detail in the RMIT Techniques Manual, or other such as the Manual of Clinical
microbiology, or Melbourne University Techniques Manual should be simply
referenced by giving the source and page number (Vancouver style, include a
page number in references to Technical manuals). The only specific materials you
need to report are kits or commercial test kits that were used, these should be
named and the manufacturer should be stated.
The simplest way to present this information is in a simple paragraph, or dot- point
format for example:
Eg. The following tests were used to identify organisms in this study. Methods were
as described in the RMIT Techniques Manual (1) or the Manual of Clinical
Microbiology (2) as indicated.
For organism A the following tests were used A:
• Catalase test (1)p24
• DNAse test (1)p56
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